anti na k atpase nka α subunit α5 antibody  (Developmental Studies Hybridoma Bank)

Journal: Marine Biotechnology (New York, N.y.)

Article Title: Epidermal distribution of tetrodotoxin-rich cells in newly hatched larvae of Takifugu spp.

doi: 10.1007/s10126-024-10377-x

Figure Lengend Snippet: Ionocyte location in larvae (0 dph) of tiger puffer Takifugu rubripes and grass puffer Takifugu alboplumbeus . Panels in A : NKA, IHC with anti-NKA antibody (yellow); WGA, fluorescence from WGA staining (green); NC, negative control; MERGE, merged image with NKA (or NC) and WGA. Panels in B : NKA, whole-mount IHC with anti-NKA antibody (yellow); WGA, WGA staining (green); DAPI, DAPI staining (blue); BF, bright-field observation. Opening of the ionocyte has no positive reaction to DAPI (white arrowheads). Scale bars: panels in A , 0.5 mm; panels in B , 20 µm

Article Snippet: VectaFluor Excel Amplified Anti-Mouse IgG, DyLight 594 Antibody Kit (containing normal horse serum, secondary antibody, and VectaFluor Reagent) and VECTASHIELD ® VibranceTM Antifade Mounting Medium were obtained from Vector Laboratories, Inc. (CA, USA) and the anti-Na + /K + -ATPase (NKA) α-subunit (α5) antibody from the Developmental Studies Hybridoma Bank (IA, USA).

Techniques: Fluorescence, Staining, Negative Control

Journal: Frontiers in Insect Science

Article Title: Protein localization of aquaporins in the adult female disease vector mosquito, Aedes aegypti

doi: 10.3389/finsc.2024.1365651

Figure Lengend Snippet: Localization of AaAQP1, 2, and 6 in whole body adult female Ae. aegypti. Immunohistochemical localization of water selective AaAQPs (red staining) in female Ae. aegypti mosquitoes (~10–12 days old). Each AaAQP (red staining) was localized under non-blood fed conditions, 0.5hr post blood meal conditions, and 24hr post blood meal conditions. Whole body sections of the abdominal segment immunolocalizing AaAQP1 in (A–C) , AaAQP2 in (D–F) , and AaAQP6 in (G–I) . In (B) , an inset image (B’) shows AaAQP1 membrane localization in the HG. In (E) , an inset image (E’) shows AaAQP2 membrane localization in the MG. All images were taken at 10x magnification (n=3). The Na + /K + -ATPase (NKA) was used as a membrane marker (green staining) and nuclei were stained with DAPI (blue). MT, Malpighian tubules; FB, fat body; OV, ovaries; HG, hindgut; MG, midgut; RP, rectal pads. White arrows indicate aggregated staining of AaAQP in the MTs.

Article Snippet: WB tissue sections were also probed with a mouse monoclonal anti-a5 antibody for Na+/K+-ATPase (NKA) (Douglas Fambrough, Developmental Studies Hybridoma Bank, IA, USA, 1:10 dilution) as a membrane marker.

Techniques: Immunohistochemical staining, Staining, Membrane, Marker

Journal: Frontiers in Insect Science

Article Title: Protein localization of aquaporins in the adult female disease vector mosquito, Aedes aegypti

doi: 10.3389/finsc.2024.1365651

Figure Lengend Snippet: Localization of AaAQP4 and 5 in whole body adult female Ae. aegypti. Immunohistochemical localization of entomoglyceroporin AaAQPs (red staining) in female Ae. aegypti mosquitoes (~10–12 days old). AaAQP4 and 5 (red staining) were localized under non-blood fed conditions, 0.5hr post blood meal conditions, and 24hr post blood meal conditions. Whole body sections of the abdominal segment immunolocalizing AaAQP4 in (A–C) with basolateral membrane of MTs encircled in dashed line and AaAQP5 in (D–F) . All images were taken at 10x magnification (n=3). The Na + /K + -ATPase (NKA) was used as a membrane marker (green staining) and nuclei were stained with DAPI (blue). MT, Malpighian tubules; FB, fat body; OV, ovaries; HG, hindgut; MG, midgut; RP, rectal pads. White arrows indicate aggregated staining of AaAQP in the MTs.

Article Snippet: WB tissue sections were also probed with a mouse monoclonal anti-a5 antibody for Na+/K+-ATPase (NKA) (Douglas Fambrough, Developmental Studies Hybridoma Bank, IA, USA, 1:10 dilution) as a membrane marker.

Techniques: Immunohistochemical staining, Staining, Membrane, Marker

Journal: Frontiers in Insect Science

Article Title: Protein localization of aquaporins in the adult female disease vector mosquito, Aedes aegypti

doi: 10.3389/finsc.2024.1365651

Figure Lengend Snippet: Localization of AaAQP1 in Malpighian tubules of adult female Ae. aegypti. Immunohistochemical localization of AaAQP1 (red staining) in isolated Malpighian tubules (MTs) of female Ae. aegypti (~10–12 days old) MTs were isolated from non-blood fed mosquitoes, and mosquitoes that were fed blood either 0.5hr or 24hr prior. (A–C) shows localization of AaAQP1 at the apical membrane of the MTs. White arrows indicate the apical membrane of the MTs. (D–F) shows localization of AaAQP1 at the apical membrane of the MTs, merged with staining observed for V-type H + -ATPase (VA), which was used as an apical membrane marker (green staining). Co-localization of AaAQP1 with VA appears yellow/orange in colour. (G–I) shows brightfield images of MT sections, which was used to identify apical and basolateral membranes. All images were taken at 20x magnification (n=4) and nuclei were stained with DAPI (blue).

Article Snippet: WB tissue sections were also probed with a mouse monoclonal anti-a5 antibody for Na+/K+-ATPase (NKA) (Douglas Fambrough, Developmental Studies Hybridoma Bank, IA, USA, 1:10 dilution) as a membrane marker.

Techniques: Immunohistochemical staining, Staining, Isolation, Membrane, Marker